![]() ![]() In this study, various chemical and nonchemical reagents were used to eliminate residual DNA within used columns from PCR purification and gel extraction kits. Thus, it is important to develop a novel method that can reduce the utilization of silica columns but not affect research efficiency. However, the use of these columns can generate plastic waste that has an environmental impact due to their one-off design and massive consumption. Silica columns from PCR purification and gel extraction kits are widely used in laboratories worldwide to assist in gene cloning.
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